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Designed for High-Concentration EPO Samples
EPO ELISA · EP100
Human Erythropoietin (EPO) ELISA Kit
The Human EPO ELISA Kit utilizes a high-specificity monoclonal antibody sandwich ELISA for quantitative determination of human erythropoietin (EPO) in serum, plasma, cell culture supernatants, and related samples. It is particularly suitable for biopharmaceutical process development, in-process monitoring, and quality control (QC) of recombinant EPO production. The pre-coated microplate format provides a simple workflow, excellent reproducibility, and reliable performance for routine laboratory testing.
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Optimized for Biopharmaceutical Quality Control
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Reliable Supply with Outstanding Cost Efficiency
Sandwich ELISA principle
The assay employs a high-specificity monoclonal antibody sandwich ELISA to specifically capture and quantify human EPO. The color intensity is proportional to the EPO concentration and is converted into sample concentration using the standard curve.
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Specific EPO Capture
A pre-coated monoclonal antibody binds human EPO in standards or samples.
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Sandwich Complex Formation
An HRP-conjugated detection antibody binds the captured EPO to form a stable sandwich immune complex.
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Color Development & Quantification
TMB substrate reacts with HRP to generate a color signal. Absorbance is measured at 450 nm and EPO concentration is calculated from the standard curve.
Assay Workflow
The kit features a streamlined five-step sandwich ELISA workflow for reliable quantitative analysis of standards and samples.
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Add standards or samples
Load 100 μL per well and incubate for 1 hour at room temperature.
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Detection Antibody Binding
Add HRP-conjugated detection antibody and incubate for 1 hour.
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Wash
Remove unbound components through four wash cycles.
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Color Development
Add TMB substrate and incubate for 15 minutes.
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Stop & Read
Add Stop Solution and immediately measure absorbance at 450 nm.
Performance Validation
Validated for precision and specificity, EP100 delivers reliable and reproducible quantification of human EPO.
Intra-assay precision
CV < 10%
Excellent repeatability within the same assay.
Inter-assay precision
CV < 10%
Consistent performance across different assay runs.
High Specificity
No detectable cross-reactivity
Specific monoclonal antibodies minimize non-specific interference.
Standard Curve & Quantification
- Subtract the absorbance value of the 0 IU/mL blank standard from all standards and samples.
- Generate the standard curve using EPO standards and calculate sample concentrations accordingly.
- A 4-parameter logistic (4PL) model is recommended. For diluted samples, multiply the calculated value by the dilution factor to obtain the final EPO concentration.
1-on-1 Expert Support
Get personalized guidance from our technical experts to help solve complex research challenges, so you can stay focused on your science.
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Chat with us
Send us an email: info@ezassay.com
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Frequently Asked Questions
Answers to common questions about ELISA product selection, assay setup, and result analysis.
Is a new standard curve required for every assay?
Yes. To maintain quantitative accuracy across reagent lots, instruments, and assay conditions, generate a standard curve with the supplied standards in every assay run.
What should I do if a sample is above 100 IU/mL?
Dilute the sample appropriately with the assay buffer and repeat the assay. Multiply the calculated concentration by the applicable dilution factor.
Is a four-parameter model mandatory for curve fitting?
No. A four-parameter model, linear regression, log-transformed linear regression, or another suitable method may be used. Select a model based on goodness of fit for the current data.