Residual DNA Detection

Host Cell Residual DNA Detection

Complete solutions from DNA extraction and purification to quantitative residual host cell DNA detection. Supporting CHO, Vero, and E.coli host systems for biopharmaceutical development, process optimization, and quality control applications.

H01

Magnetic Bead-Based DNA Sample Preparation

H02

CHO / Vero qPCR-Based Quantification

H03

E.coli LAMP-Based Quantification

Why Choose EZassay Residual Host Cell DNA Detection Solutions?

EZassay provides complete solutions from sample preparation and DNA purification to residual host cell DNA quantification, covering major host systems including CHO, Vero, and E.coli. By combining industry-established qPCR assays with innovative LAMP-based quantification technology, EZassay delivers sensitive and reliable solutions for biopharmaceutical development, process optimization, and quality control applications.

  • Industry-Compatible qPCR Detection Platform: CHO and Vero residual DNA assays utilize real-time qPCR technology, ensuring compatibility with established workflows without changing existing laboratory procedures.
  • High-Sensitivity LAMP Quantification Platform: The EZassay E.coli residual DNA detection kit utilizes fluorescence-based quantitative LAMP technology, achieving a broad linear detection range of 3 fg/mL–300 pg/mL for sensitive quantification of low-abundance residual DNA.
  • Rapid and Reliable QC Workflow: The isothermal amplification workflow simplifies testing procedures, shortens turnaround time, and supports high-frequency quality control and routine process monitoring.
  • Cost-Effective Detection Solution: Delivers comparable performance to leading international solutions while reducing testing costs and improving quality control efficiency.

Complete Workflow for Residual Host Cell DNA Detection

EZassay provides an integrated workflow covering sample DNA extraction, purification, and residual host cell DNA quantification. Detection methods can be selected according to different host cell sources, enabling standardized and reliable quality control analysis.

01

Sample DNA Extraction and Purification

  1. STEP 01

    Prepare Samples and Controls

    Determine the dilution according to the sample matrix. Process each sample ERC together with its corresponding test sample.

  2. STEP 02

    Magnetic-Bead Extraction and Purification

    Follow the sample preparation kit instructions for lysis, magnetic-bead enrichment, washing, and elution.

  3. STEP 03

    Collect Purified DNA

    Use the purified DNA eluate as the template for the corresponding host-cell residual DNA assay.

02

Select the Appropriate Host DNA Detection Assay

SYSTEM 01

CHO

qPCR Quantification

For CHO host-cell residual DNA, quantified with a standard curve.

Assay Conditions

qPCR reaction master mix + template.

QC Setup

Standard curve (at least 5 points) · NTC · NEC · Sample ERC

SYSTEM 02

Vero

qPCR Quantification

For Vero host-cell residual DNA, quantified with a standard curve.

Assay Conditions

qPCR reaction master mix + template.

QC Setup

Standard curve (at least 5 points) · NTC · NEC · Sample ERC

SYSTEM 03

E.coli

LAMP Quantification

For E.coli host-cell residual DNA, using an isothermal fluorescent-probe assay.

Assay Conditions

LAMP reaction master mix + template.

QC Setup

Standard curve (at least 5 points) · NTC · NCS · Sample ERC

03

Result Interpretation and Quality Control

  • CHOqPCR Quantification
    • NTC: Monitor contamination during amplification
    • NEC: Monitor contamination during sample extraction
    • ERC: Evaluate DNA extraction recovery efficiency
    • Calculate Residual DNA Concentration Based on the Valid Standard Curve
  • VeroqPCR Quantification
    • NTC: Monitor contamination during amplification
    • NEC: Monitor contamination during sample extraction
    • ERC: Evaluate DNA extraction recovery efficiency
    • Calculate Residual DNA Concentration Based on the Valid Standard Curve
  • E.coliLAMP Quantification
    • NTC: Monitor contamination during amplification
    • NEC: Monitor contamination during sample extraction
    • ERC: Evaluate DNA extraction recovery efficiency
    • Calculate Residual DNA Concentration Based on the Valid Standard Curve

Residual Host Cell DNA Detection Platform Comparison

EZassay offers tailored quantitative residual host cell DNA detection solutions for different host systems, including CHO and Vero qPCR assays, E.coli LAMP assays, and integrated magnetic bead-based DNA sample preparation workflows.

ItemCHO Residual DNA DetectionVero Residual DNA DetectionE.coli Residual DNA Detection
Detection MethodqPCRqPCRQuantitative LAMP
Target Host DNACHO Host Cell DNAVero Host Cell DNAE.coli Host Cell DNA
Detection Range3 fg/μL – 300 pg/μL3 fg/μL – 300 pg/μL3 fg/μL – 300 pg/μL
Quality ControlStandard Curve, NTC, NEC, ERCStandard Curve, NTC, NEC, ERCStandard Curve, NTC, NEC, ERC

1-on-1 Expert Support

Get personalized guidance from our technical experts to help solve complex research challenges, so you can stay focused on your science.

01

Chat with us

Send us an email: info@ezassay.com

02

Call us

Call our team Mon–Fri from 8am to 5pm.

+86 19925271988
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