NUCLEIC ACID TEST STRIPS

Nucleic Acid Test Strips

Based on lateral flow technology, EZassay enables rapid visual readout of RPA, LAMP, and CRISPR-based detection results. Multiple formats including single-target, dual-target, line-appearance, and line-disappearance detection are available for molecular diagnostic development.

H01

Multi-platform Compatibility: Compatible with RPA, LAMP, and CRISPR SHERLOCK workflows.

H02

Rapid Visual Readout: Results available within 5–15 minutes without specialized equipment.

H03

Flexible Detection Formats: Supports single-target, dual-target, and multiple CRISPR readout formats.

Why Choose EZassay Nucleic Acid Test Strips?

Based on lateral flow technology, EZassay nucleic acid test strips provide rapid and reliable visual readout solutions for RPA, LAMP, and CRISPR-based nucleic acid detection platforms. With flexible labeling strategies and multiple detection formats, EZassay enables convenient applications from laboratory research to field-based rapid testing.

  • 01

    Multiple Amplification Compatibility: Compatible with RPA, LAMP, and other isothermal amplification technologies for rapid visual readout.

  • 02

    Flexible CRISPR Detection Formats: Supports Cas12, Cas13, and other CRISPR-based nucleic acid detection workflows.

  • 03

    Instrument-Free Visual Readout: Direct visual interpretation without fluorescence equipment, enabling rapid point-of-care testing.

  • 04

    Closed-Tube Workflow for Contamination Prevention: Customized consumables minimize opening steps after amplification and reduce aerosol contamination risks.

Nucleic Acid Test Strip Principle & Result Interpretation

Based on lateral flow technology, nucleic acid test strips generate visual signals by specifically recognizing labeled amplification products or CRISPR-generated reporter signals. Different detection formats produce distinct changes at the test line (T line) and control line (C line), enabling rapid result interpretation.

RPA / LAMPFAM / FITC + BiotinT / C readout

Nucleic Acid Test Strip

FAM/FITC + Biotin dual-labeled amplification products with T/C line readout

Operating Conditions

Dilute 10 µL amplicon 10-20 fold with Diluent Buffer; load 70 µL and read within 5 minutes.

Result Interpretation

  • Negative: C line appears and T line is absent.
  • Positive: both C and T lines appear.
  • Invalid: C line is absent; repeat the test.
Working principle diagram for RPA or LAMP nucleic acid test strips
Duplex target detectionT1 / T2 / C

Dual-Test Line Nucleic Acid Test Strip

T1/T2 dual test lines for simultaneous dual-target detection

Operating Conditions

Mix 5 µL amplicon with 100 µL Diluent Buffer; load 80 µL and read within 15 minutes.

Result Interpretation

  • Negative: C line appears and T1 / T2 are absent.
  • Target 1 positive: C and T1 lines appear; target 2 positive: C and T2 lines appear.
  • Duplex positive: C, T1, and T2 lines all appear; T1 / T2 intensity does not change the positive result.
  • Invalid: C line is absent. Repeat the test regardless of T1 / T2 status.
Principle diagram for duplex nucleic acid test strips
CRISPR SHERLOCKDual-labeled reporterPositive line appearance

CRISPR SHERLOCK Test Strip (Direct Format)

T-line appearance upon CRISPR activation

Operating Conditions

Mix 10 µL CRISPR-Cas reaction product with 40 µL ddH₂O. Insert the strip, keep liquid below the MAX line, and read after 5-10 minutes.

Result Interpretation

  • Negative: T line is absent, indicating that the reporter was not cleaved by Cas.
  • Positive: a visible T line indicates Cas activation.
  • Invalid: both C and T lines are absent; repeat the test.
Principle diagram for CRISPR SHERLOCK nucleic acid test strips
CRISPR SHERLOCKLine-disappearance methodPositive T line absent

CRISPR SHERLOCK Line-Disappearance Test Strip (Indirect Format)

Target-triggered Cas activation cleaves the reporter, resulting in T-line disappearance

Operating Conditions

Mix 2 µL amplicon with 78 µL Diluent Buffer; load 70 µL and read within 5 minutes.

Result Interpretation

  • Negative: both T and C lines appear.
  • Positive: T line is absent and C line appears.
  • Invalid: C line is absent, or both C and T are absent; repeat the test.
Principle diagram for CRISPR SHERLOCK line-disappearance test strips

Nucleic Acid Test Strip Selection Guide

Product NameCompatible PlatformRecommended ApplicationKey Features
Nucleic Acid Test StripRPA/LAMPSingle–Target DetectionSimple workflow for rapid visual detection of amplification products
Dual–Test Line Nucleic Acid Test StripRPA/LAMPDual–Target DetectionEnables simultaneous detection of two targets, increasing detection capacity and application flexibility
CRISPR SHERLOCK Test Strip (Direct Format)Cas12a/Cas13aDirect Visual CRISPR DetectionGenerates visual signals upon CRISPR activation, providing intuitive readout for rapid assay development
CRISPR SHERLOCK Line–Disappearance Test Strip (Indirect Format)Cas12a/Cas13aDirect Visual CRISPR DetectionPositive results are determined by T–line disappearance, with sufficient reaction time to improve detection stability

1-on-1 Expert Support

Get personalized guidance from our technical experts to help solve complex research challenges, so you can stay focused on your science.

01

Chat with us

Send us an email: info@ezassay.com

02

Call us

Call our team Mon–Fri from 8am to 5pm.

+86 19925271988
Product of Interest